The Notch signaling pathway is highly conserved in genetic evolution, and the Notch receptor family in mammals includes four receptors (Notch 1-4), five corresponding Notch ligands (Jagged1-2 and Delta-like1, Delta-like3, and Delta-4), all of which are widely expressed on the surface of various cells ( Luca et al., 2017). However, Eg.mMDH stimulated DCs to produce an immune response showing weak interference by DAPT and Jagged1.ĭiscussion: The study suggests that the Notc h signaling pathway is involved in the Eg.ferritin-sensitized DC-mediated immune response, which may become a new target for treating E.granulosus infection. Following Jagged1 addition, the function of DCs was restored to some extent, and the expression of Notch1, Delta4 and Hes1 was activated in response to the stimulation of Eg.ferritin. Results: DAPT inhibited the development and maturation of DCs, which were in a non-responsive or incompetent state, reduced the sensitization of DCs to Eg.ferritin, weakened the migration ability of DCs, disrupted their ability to mediate T-cell proliferation, reduced DC expression of MHCII, CD80, CD60, and CD40 co-stimulatory molecules, prevented the secretion of cytokines and attenuated the expression of Notch1, Notch2, Notch3 receptors, Jagged1, Delta-like 4 (Delta4), and Hes1.
granulosus antigens and the mediated immune response. We observed the changes in DC response to E. We analyzed the effect of Notch signaling on the development and differentiation of DCs by detecting their morphology, migration function, capacity to promote T cell proliferation, and cytokine secretion. Methods: We used the Notch signaling pathway inhibitor -L-alanyl]-S-phenylglycinet-butyl ester (DAPT) or activator Jagged1 to construct in vitro cell models with blocked or activated Notch signaling respectively. granulosus antigens, and clarified whether it is involved in E.granulosus infection. Therefore, in the study we explored whether the Notch signaling pathway affects the development and differentiation of DCs, causing changes in the immune response of DCs sensitized with E. We previously found that ferritin and malate dehydrogenase (mMDH) in Echinococcus granulosus (E.granulosus) induced different immune responses through sensitized DCs.
Introduction: The Notch signaling pathway is involved in the development of many diseases it regulates the development of dendritic cells (DCs), and affects the immune response of DC-mediated T cells.
1Basic Medical Institute of Ningxia Medical University, Yinchuan, China.Mingxia Wang 1, Zailing Shang 1, Fei Qiao 1, Junhu Hei 1, Xueling Ma 1 and Yana Wang 1,2*
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